Overview
The method used is under flexible scope ISO17025 for food and feed samples using Dispersive Solid Phase Extraction (DSPE) followed by LC-MS/MS. The method was fully validated by an international validation study run by Fera and is the basis of the standard method BS EN 17425:2021.
The method is capable of measuring the main Claviceps purpurea ergot alkaloids, namely ergometrine, ergotamine, ergosine, ergocristine, ergocryptine, ergocornine, as well as the corresponding –inine epimers.
Commission Regulation (EU) 2021/1399 was published on 24 August 2021 amending Regulation (EC) No 1881/2006 and introducing maximum levels of ergot sclerotia and ergot alkaloids in certain foodstuffs. The limits will apply in the EU from 1 January 2022.
Parameters Tested
We analyse for the following 12 parameters:
Ergocornine, Ergocorninine, Ergocristine, Ergocristinine, Ergocryptine, Ergocryptinine, Ergometrine, Ergometrinine, Ergosine, Ergosinine, Ergotamine, Ergotaminine
Reporting limit
0.5 µg/kg each analyte, (6 µg/kg sum ergot alkaloids)
| Detail | Specification |
|---|---|
| Method Used | LC-MS/MS |
| Accreditation | ISO17025 |
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Ergot alkaloids
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